Seldi
SELDI-TOF
MS: How much does it cost?
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| It is known that the expenses for performing proteomics are high. This is also the case for profiling experiments, as it requires several ProteinChip arrays and skilled expertise. The costs not only depend on the materials but also largely depend on the service required. In general all liquid handling procedures have to be performed by the researcher. The operator is involved in the analysis of the chip and the first evaluation of the results. For proper statistical analysis further expertise is required. |
| Expression Difference Mapping SELDI-TOF experiments reuires two different stages. The first part deals with searching the most optimal binding conditions for profiling. The researcher can decide with conditions have to be tested. The experiments should be performed with some test samples. The second part is to test the whole study population with the optimal binding condition(s). |
| For performing Interaction Difference Mapping SELDI-TOF the "Bait" molecules have to coupled covalently to the preactivated chip. Several preactivated chips are currently available and it should be tested which preactivated chip allows the best coupling. The second part is similar to the one described above in the EDM SELDI-TOF approach. |
| In general the most expensive part of the analysis is the exploring part defing the most optimal binding conditions (part 1). Once the optimal conditions have been established the whole study population can be analysed in a routine fashion. |
| If you like to get more information on the analysis costs, please contact: |
| Dr.
Waander van Heerde e-mail: w.vanheerde@chl.umcn.nl tel: 024-3610800 |
