FTMS
			Sample requirements
			Samples can be submitted in two formats, either as a gel slice or spot, or as a solution of proteins. There are specific requirements for both formats, but a few requirements are general: The sample amount should be more than 100 fmole The genome of the species from which the sample originates has to be sequenced and annotated No radioactive samples ! Sample volume smaller than 100 ul << Back Requirements for a gel slice or spot Gel can be stained with either coomassie, colloidal coomassie or silver staining. In case of silver staining, the staining has to be performed according to our protocol (look at section protocols). Be careful for contamination with keratins from skin or hair. Wear gloves, never touch the gel with bare hands and use clean glass plates and scalpels for excision of bands. Gel bands or spots can be submitted in a simple eppendorf tube. The band can be stored dry at -20 ºC. A picture of the gel from which the band is excised should be included. Please circle the bands of interest.  << Back Requirements for a solution of proteins The requirements are depending on the goal of the experiment. Requirements for in solution digestion The sample should be absolutely free of detergents Salt concentration should be < 500 mM Glycerol concentration should be < 2,5% No peptide elution No protease inhibitors No organic solvents Sample is preferably solved in 8M ureum/thioureum Sample can be submitted in dry form, either freeze dried of vacuum dried Requirements for fractionation on 1D gel electrophoresis Salt concentration should be < 500 mM Ureum concentration should be < 0,5 M << Back