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Part of the Nijmegen Proteomics Facility is the
FTMS facility. At this site, a nano-LC system is online coupled with an
advanced, high-performance mass spectrometer: the LTQ Fourier Transform Ion
Cyclotron Resonance Mass spectrometer. Its ultra-high mass resolution, mass
accuracy and sensitivity make it a powerful solution for your complex
proteomics problems.
Because of the one dimensional or two dimensional
nanoscale chromatography, even highly complex samples can be analyzed.
Protein samples will be treated with proteolytic enzymes to yield peptides.
These peptides can be separated on the LC system and the eluting peptides
will be directly measured in FTMS mass spectrometer. Accurate mass
determination and MS/MS on the peptides will allow efficient database
searches. Automatic or manual verification of the dataset results in a list
of peptides or proteins that was present in the sample.
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