nanoLC-MS/MS

About nanoLC-MS/MS

The configuration we’re using is a LTQ FT Ultra from Thermo Fisher Scientific, coupled to a nanoLC system (1D nanoLC (Proxeon, Easy-nLC) or 2D nanoLC (Agilent 1100 series)). The mass spectrometer consists of two main parts: the LTQ, which is a linear ion trap mass spectrometer, and the Fourier Transform Ion Cyclotron Resonance (FT-ICR) mass spectrometer.

The instrument can perform fragmentation analyses using different dissociation modes: Collision Induced Dissociation (CID) in the LIT, Electron Capture Dissociation (ECD) and Infrared Multi Photon dissociation (IRMPD) in the FT-ICR cell.

With these different fragmentation techniques at our disposal, the configuration becomes exquisitely suited for the analysis of post translational modifications.

	Animation of ions in the ion trap
	Animation of ions in the FTMS