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Nijmegen Proteomics Facility also offers high quality two-dimensional
electrophoresis. Two-dimensional electrophoresis is still a protein
separation method of choice in proteomics because of its high resolving
capabilities and reproducibility. Electrophoretic separation of proteins
in two-dimensional space offers the possibility to unambiguously analyse
proteins, (post-translational) protein modifications, and native protein
complexes.
The analysis of proteins
of interest can be addressed on the qualitative or quantitative
level and offers the user an intuitive way for obtaining the desired
information. Besides conventional 2D electrophoresis we also offer
the possibility of two-dimensional BlueNative electrophoresis for
the analysis of native protein complexes and membrane proteins.
Another topic is post-translational modifications, which are often
difficult to analyse at the proteome level. For a number of modifications
new possibilities are available via highly selective staining methods
that specifically stain proteins that carry the post-translational
modifications of interest.
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